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transcription factor 6  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc transcription factor 6
    Transcription Factor 6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 383 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcription+factor+6/ATF-6+Rabbit+mAb/pm41161548-111-41-48
    Average 96 stars, based on 383 article reviews
    transcription factor 6 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Ligustilide Improves Cognitive Impairment via Regulating the SIRT1/IRE1 α /XBP1s/CHOP Pathway in Vascular Dementia Rats.
    Article Snippet: EX527 (CAS: 49843-98-3) was purchased from MedChemExpress. .. Primary antibodies of Bax (CAS: 2772s), Bcl2 (CAS: 7498s), sirtuin-1 (SIRT1, CAS: 9475s), binding immunoglobulin protein (BIP, CAS: 3177s), protein disulfide isomerase (PDI, CAS: 3501s), protein kinase R-like endoplasmic reticulum kinase (PERK, CAS: 3192s), phospho-protein kinase R-like endoplasmic reticulum kinase (P-PERK, CAS: 3179s), inositol-requiring enzyme1α (IRE1α, CAS: 3294s), activating transcription factor 6 (ATF6 CAS: 65880s), and C/EBP-homologous protein (CHOP, CAS: 2895) were purchased from Cell Signaling Technology, Inc. Primary antibodies of β-actin (CAS: ab8227) and X-box binding protein 1 (XBP1, CAS: ab37152) were purchased from Abcam, Inc. .. The primary antibody phospho-inositol-requiring enzyme-1α (P-IRE1α, CAS: NB100-2323) was purchased from Novus Biologicals.

    Article Title: Nicotine exacerbates tacrolimus-induced renal injury by programmed cell death.
    Article Snippet: TAC (Prograf, Astellas Pharma, Ibaraki, Japan) and NIC (Sigma-Aldrich) doses were based on previous studies [9,10]. .. The antibodies used in this study for immunohistochemistry and immunoblotting were as follows: TGF-β1 (R&D Systems, Minneapolis, MN, USA), connective tissue growth factor (CTGF, #ab125943, Abcam, Cambridge, UK), TGF-β inducible gene-h3 (βig-h3, Proteintech, Chicago, IL, USA), ectodermal dysplasia-1 (ED-1, Serotec Inc., Oxford, UK), monocyte chemotactic protein-1 (MCP-1, Santa Cruz Biotechnology, Dallas, TX, USA), interleukin-1beta (IL-1β, #ab9722, Abcam), interleukin 18 (IL-18, #ab191152, Abcam), NOD-like receptor pyrin domain-containing protein 3 (NLRP3, #ab214185, Abcam), superoxide dismutase 1 (SOD1, #ab16831, Abcam), superoxide dismutase 2/manganese superoxide dismutase (SOD2/MnSOD, #ab13534, Abcam), NADPH oxidase-2 (NOX-2, #ab31092, Abcam), NADPH oxidase-4 (NOX-4, #ab109225, Abcam), B-cell lymphoma-2 (Bcl2, #ab59348, Abcam), Bcl2-associated X (Bax, #ab32503, Abcam), cleaved caspase-3 (Millipore, Billerica, MA, USA), p62 (#ab56416, Abcam), light chain 3B (LC3B, #ab192890, Abcam), Parkin (#2132, Cell Signaling, Danvers, MA, USA), phosphate and tension homology deleted on chromsome ten-induced kinase 1 (PINK1, N4/15, #ab186303, Abcam), optic atrophy protein 1 (OPA1, #ab42364, Abcam), dynamin-related protein 1 (DLP1/ Drp1, BD Transduction Laboratories, Lexington, KY, USA), C/EBP homologous protein (CHOP, L63F7, #2895, Cell Signaling), binding immunoglobulin protein (BiP, C50B12, Rabbit mAb, #3177, Cell Signaling), inositol-re- 1439 www.kjim.orghttps://doi.org/10.3904/kjim.2021.326 quiring protein-1α (IRE-1α, phospho S724, #ab48187, Abcam), activating transcription factor 6 (ATF-6, D4Z8V, Rabbit mAb, #65880, Cell Signaling), and β-actin (#ab8226, Abcam). ..

    Article Title: Omentin-1 protects against high glucose-induced endothelial dysfunction via the AMPK/PPARδ signaling pathway.
    Article Snippet: High glucose-induced endothelial dysfunction is a critical initiating factor in the development of diabetic vascular complications.. Omentin-1 has been regarded as a novel biomarker of endothelial function in subjects with type-2 diabetes (T2D); however, it is unclear whether omentin-1 has any direct effect in ameliorating high glucose-induced endothelial dysfunction.. In the present study, we analyzed the effect of omentin-1 on high glucose-induced endothelial dysfunction in isolated mouse aortas and mouse aortic endothelial cells (MAECs).

    Immunohistochemistry:

    Article Title: Nicotine exacerbates tacrolimus-induced renal injury by programmed cell death.
    Article Snippet: TAC (Prograf, Astellas Pharma, Ibaraki, Japan) and NIC (Sigma-Aldrich) doses were based on previous studies [9,10]. .. The antibodies used in this study for immunohistochemistry and immunoblotting were as follows: TGF-β1 (R&D Systems, Minneapolis, MN, USA), connective tissue growth factor (CTGF, #ab125943, Abcam, Cambridge, UK), TGF-β inducible gene-h3 (βig-h3, Proteintech, Chicago, IL, USA), ectodermal dysplasia-1 (ED-1, Serotec Inc., Oxford, UK), monocyte chemotactic protein-1 (MCP-1, Santa Cruz Biotechnology, Dallas, TX, USA), interleukin-1beta (IL-1β, #ab9722, Abcam), interleukin 18 (IL-18, #ab191152, Abcam), NOD-like receptor pyrin domain-containing protein 3 (NLRP3, #ab214185, Abcam), superoxide dismutase 1 (SOD1, #ab16831, Abcam), superoxide dismutase 2/manganese superoxide dismutase (SOD2/MnSOD, #ab13534, Abcam), NADPH oxidase-2 (NOX-2, #ab31092, Abcam), NADPH oxidase-4 (NOX-4, #ab109225, Abcam), B-cell lymphoma-2 (Bcl2, #ab59348, Abcam), Bcl2-associated X (Bax, #ab32503, Abcam), cleaved caspase-3 (Millipore, Billerica, MA, USA), p62 (#ab56416, Abcam), light chain 3B (LC3B, #ab192890, Abcam), Parkin (#2132, Cell Signaling, Danvers, MA, USA), phosphate and tension homology deleted on chromsome ten-induced kinase 1 (PINK1, N4/15, #ab186303, Abcam), optic atrophy protein 1 (OPA1, #ab42364, Abcam), dynamin-related protein 1 (DLP1/ Drp1, BD Transduction Laboratories, Lexington, KY, USA), C/EBP homologous protein (CHOP, L63F7, #2895, Cell Signaling), binding immunoglobulin protein (BiP, C50B12, Rabbit mAb, #3177, Cell Signaling), inositol-re- 1439 www.kjim.orghttps://doi.org/10.3904/kjim.2021.326 quiring protein-1α (IRE-1α, phospho S724, #ab48187, Abcam), activating transcription factor 6 (ATF-6, D4Z8V, Rabbit mAb, #65880, Cell Signaling), and β-actin (#ab8226, Abcam). ..

    Western Blot:

    Article Title: Nicotine exacerbates tacrolimus-induced renal injury by programmed cell death.
    Article Snippet: TAC (Prograf, Astellas Pharma, Ibaraki, Japan) and NIC (Sigma-Aldrich) doses were based on previous studies [9,10]. .. The antibodies used in this study for immunohistochemistry and immunoblotting were as follows: TGF-β1 (R&D Systems, Minneapolis, MN, USA), connective tissue growth factor (CTGF, #ab125943, Abcam, Cambridge, UK), TGF-β inducible gene-h3 (βig-h3, Proteintech, Chicago, IL, USA), ectodermal dysplasia-1 (ED-1, Serotec Inc., Oxford, UK), monocyte chemotactic protein-1 (MCP-1, Santa Cruz Biotechnology, Dallas, TX, USA), interleukin-1beta (IL-1β, #ab9722, Abcam), interleukin 18 (IL-18, #ab191152, Abcam), NOD-like receptor pyrin domain-containing protein 3 (NLRP3, #ab214185, Abcam), superoxide dismutase 1 (SOD1, #ab16831, Abcam), superoxide dismutase 2/manganese superoxide dismutase (SOD2/MnSOD, #ab13534, Abcam), NADPH oxidase-2 (NOX-2, #ab31092, Abcam), NADPH oxidase-4 (NOX-4, #ab109225, Abcam), B-cell lymphoma-2 (Bcl2, #ab59348, Abcam), Bcl2-associated X (Bax, #ab32503, Abcam), cleaved caspase-3 (Millipore, Billerica, MA, USA), p62 (#ab56416, Abcam), light chain 3B (LC3B, #ab192890, Abcam), Parkin (#2132, Cell Signaling, Danvers, MA, USA), phosphate and tension homology deleted on chromsome ten-induced kinase 1 (PINK1, N4/15, #ab186303, Abcam), optic atrophy protein 1 (OPA1, #ab42364, Abcam), dynamin-related protein 1 (DLP1/ Drp1, BD Transduction Laboratories, Lexington, KY, USA), C/EBP homologous protein (CHOP, L63F7, #2895, Cell Signaling), binding immunoglobulin protein (BiP, C50B12, Rabbit mAb, #3177, Cell Signaling), inositol-re- 1439 www.kjim.orghttps://doi.org/10.3904/kjim.2021.326 quiring protein-1α (IRE-1α, phospho S724, #ab48187, Abcam), activating transcription factor 6 (ATF-6, D4Z8V, Rabbit mAb, #65880, Cell Signaling), and β-actin (#ab8226, Abcam). ..

    Article Title: Chronic hypoxia or MitoQ treatment in pregnancy independently alter hepatic cytochrome P450 activity in fetal sheep and 9-month-old lambs.
    Article Snippet: .. Membranes were then blocked in 5 % bovine serum albumin (BSA) in TBS with 1 % Tween-20 (TBS-T) for 1 h at room temperature and underwent three 5 min washes in TBS-T before being incubated overnight with their respective antibody: Activating Transcription Factor 6 (ATF6; 1:500, D4Z8V, #65880, Cell Signaling Technology, Massachusetts, USA), Catalase (CAT; 1:1000, D5N7V, #14097, Cell Signaling Technology, Massachusetts, USA), Hepatocyte nuclear factor 4 alpha (HNF4ɑ; c-19, 1:1000, sc-6556, Santa Cruz Biotechnology, Texas, USA), MitoBiogenesis Western Blot Cocktail (ab123545, Abcam, Cambridge, UK), Peroxisome proliferator activated receptor alpha (PPARɑ; 1:1000, sc-9000, Santa Cruz Biotechnology, Texas, USA), Superoxide dismutase (SOD; 1:1000, #06–984, Merck, Germany), Total OXPHOS Antibody Cocktail (ab110413, Abcam, Cambridge, UK). ..

    Blocking Assay:

    Article Title: Omentin-1 protects against high glucose-induced endothelial dysfunction via the AMPK/PPARδ signaling pathway.
    Article Snippet: High glucose-induced endothelial dysfunction is a critical initiating factor in the development of diabetic vascular complications.. Omentin-1 has been regarded as a novel biomarker of endothelial function in subjects with type-2 diabetes (T2D); however, it is unclear whether omentin-1 has any direct effect in ameliorating high glucose-induced endothelial dysfunction.. In the present study, we analyzed the effect of omentin-1 on high glucose-induced endothelial dysfunction in isolated mouse aortas and mouse aortic endothelial cells (MAECs).

    Incubation:

    Article Title: Omentin-1 protects against high glucose-induced endothelial dysfunction via the AMPK/PPARδ signaling pathway.
    Article Snippet: High glucose-induced endothelial dysfunction is a critical initiating factor in the development of diabetic vascular complications.. Omentin-1 has been regarded as a novel biomarker of endothelial function in subjects with type-2 diabetes (T2D); however, it is unclear whether omentin-1 has any direct effect in ameliorating high glucose-induced endothelial dysfunction.. In the present study, we analyzed the effect of omentin-1 on high glucose-induced endothelial dysfunction in isolated mouse aortas and mouse aortic endothelial cells (MAECs).

    Article Title: Enalapril attenuates endoplasmic reticulum stress and mitochondrial injury induced by myocardial infarction via activation of the TAK1/NFAT pathway in mice.
    Article Snippet: .. Next, the membranes were blocked with 5% fat‐free milk for 2 h at 4 ̊C and incubated with the relevant protein antibodies overnight at 4 ̊C, including antibodies against poly (ADP‐ribose) polymerase (PARP)‐1 (1:1,000; cat. no. 9532, Cell Signaling Technology, Inc.), caspase‐9 (1:1,000; cat. no. 9508; Cell Signaling Technology, Inc.), cleaved caspase‐9 (1:1,000; cat. no. 7237; Cell Signaling Technology, Inc.), caspase‐3 (1:1,000; cat. no. 9665; Cell Signaling Technology, Inc.), cleaved caspase‐3 (1:1,000; cat. no. 9694; Cell Signaling Technology, Inc.), 78 kDa glucose-regulated protein (GRP78; 1:500; cat. no. sc‐13968; Santa Cruz Biotechnology, Inc.), activating transcription factor 6 (ATF6; 1:1,000; cat. no. 65880; Cell Signaling Technology, Inc.), Bcl‐2 (1:1,000; cat. no. 3498; Cell Signaling Technology, Inc.), Bax (1:1,000; cat. no. 14796; Cell Signaling Technology, Inc.), TAK1 (1:1,000; cat. no. ab109526, Abcam), phosphorylated TAK1 (1:1,000; cat. no. 9339; Cell Signaling Technology, Inc.), NFAT3 (1:1,000; cat. no. ab3347; Abcam), β-actin (1:1,000; cat. no. 3700; Cell Signaling Technology, Inc.) and Lamin B1 (1:1,000; cat. no. 13435; Cell Signaling Technology, Inc.). .. The membranes were washed with TBS containing 1% Tween-20 and incubated with horseradish peroxidase-conjugated second antibody (1:3,000; cat. no. 7074; Cell Signaling Technology, Inc.) for 2 h at room temperature.

    Article Title: Chronic hypoxia or MitoQ treatment in pregnancy independently alter hepatic cytochrome P450 activity in fetal sheep and 9-month-old lambs.
    Article Snippet: .. Membranes were then blocked in 5 % bovine serum albumin (BSA) in TBS with 1 % Tween-20 (TBS-T) for 1 h at room temperature and underwent three 5 min washes in TBS-T before being incubated overnight with their respective antibody: Activating Transcription Factor 6 (ATF6; 1:500, D4Z8V, #65880, Cell Signaling Technology, Massachusetts, USA), Catalase (CAT; 1:1000, D5N7V, #14097, Cell Signaling Technology, Massachusetts, USA), Hepatocyte nuclear factor 4 alpha (HNF4ɑ; c-19, 1:1000, sc-6556, Santa Cruz Biotechnology, Texas, USA), MitoBiogenesis Western Blot Cocktail (ab123545, Abcam, Cambridge, UK), Peroxisome proliferator activated receptor alpha (PPARɑ; 1:1000, sc-9000, Santa Cruz Biotechnology, Texas, USA), Superoxide dismutase (SOD; 1:1000, #06–984, Merck, Germany), Total OXPHOS Antibody Cocktail (ab110413, Abcam, Cambridge, UK). ..



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    Image Search Results


    Induction of ER stress in the kidney (A) Heatmap hierarchical correlation analysis of differentially expressed genes (DEGs) (absolute fold-change >1.5 and P <0.05) (left) and GSEA-enrichment plot of the Hallmark category showing the unfolded protein response (UPR) (NES = 1.583, FDR = 0.008) positively correlated with HFD+STZ treatment in kidney tissue using transcriptome data (n = 3 each) (middle). The top 20 genes comprising the enrichment score's leading edge are indicated in the corresponding heatmap (blue, low; red, high). Bar graphs (right) of significantly enriched UPR-related signaling pathways positively correlated with HFD+STZ-treated WT mice in GSEA biological process and molecular function. NES and NOM P -value are shown in the bar graph. (B) Schematic of the induction and treatment protocol. Diabetes was established by feeding mice a 60% HFD for 18 weeks, with the addition of STZ injections (50 mg/kg body weight, i.p three times) at the 12th week. The AZ2 compound (10 mg/kg body weight) was administered orally five times weekly starting at week 15 and continuing until week 18. (C) Immunodetection assays for IRE1α, ATF6, and GRP78 were done using kidney cortex (upper) and medulla (lower) homogenate samples. For C , β-actin was used as normal control for densitometric analysis. The quantification of relative band density is provided for the indicated samples (n = 4 or 5 per group). Results represent the mean ± SEM. One-way ANOVA combined with LSD post-hoc analysis was utilized to calculate significance (*p < 0.05, **p < 0.01, ***p < 0.001). Groups with no significant difference are labeled N.S. (not significant).

    Journal: Theranostics

    Article Title: Hyperglycemia in combination with excess fat intake promotes renal pyroptosis and fibrosis through Gα 12 -dependent endoplasmic reticulum stress

    doi: 10.7150/thno.124015

    Figure Lengend Snippet: Induction of ER stress in the kidney (A) Heatmap hierarchical correlation analysis of differentially expressed genes (DEGs) (absolute fold-change >1.5 and P <0.05) (left) and GSEA-enrichment plot of the Hallmark category showing the unfolded protein response (UPR) (NES = 1.583, FDR = 0.008) positively correlated with HFD+STZ treatment in kidney tissue using transcriptome data (n = 3 each) (middle). The top 20 genes comprising the enrichment score's leading edge are indicated in the corresponding heatmap (blue, low; red, high). Bar graphs (right) of significantly enriched UPR-related signaling pathways positively correlated with HFD+STZ-treated WT mice in GSEA biological process and molecular function. NES and NOM P -value are shown in the bar graph. (B) Schematic of the induction and treatment protocol. Diabetes was established by feeding mice a 60% HFD for 18 weeks, with the addition of STZ injections (50 mg/kg body weight, i.p three times) at the 12th week. The AZ2 compound (10 mg/kg body weight) was administered orally five times weekly starting at week 15 and continuing until week 18. (C) Immunodetection assays for IRE1α, ATF6, and GRP78 were done using kidney cortex (upper) and medulla (lower) homogenate samples. For C , β-actin was used as normal control for densitometric analysis. The quantification of relative band density is provided for the indicated samples (n = 4 or 5 per group). Results represent the mean ± SEM. One-way ANOVA combined with LSD post-hoc analysis was utilized to calculate significance (*p < 0.05, **p < 0.01, ***p < 0.001). Groups with no significant difference are labeled N.S. (not significant).

    Article Snippet: Primary antibodies were sourced from various suppliers as follows: α-SMA (ab7817) was obtained from Abcam (Cambridge, MA, USA); Fibronectin (610078) from BD Biosciences (Franklin Lakes, NJ, USA); and activating transcription factor 6 (ATF6) (NBD1-40256) from Novus Biologicals (Littleton, CO, USA).

    Techniques: Protein-Protein interactions, Immunodetection, Control, Labeling

    Gα 12 family overexpression in the kidney. (A) Immunodetection of Gα 12 , Gα 13 , PGC1α, and PPARα was carried out on the homogenates used in Figure , with band intensity quantification provided for n= 4 or 5 per group. (B) Representative IHC images showing Gα 12 and Gα 13 localization; scale bar represents 100 μm. (n= 4 or 5 per group) (C) Pearsons's correlation were calculated to determine the correlation between Gα 12 or Gα 13 and either IRE1α or ATF6 (n= 4 or 5/group). For A , β-actin was used as normal control for densitometric analysis. Values are represented as mean ± SEM (** p < 0.01, *** p < 0.001, and N.S., not significant). One-way ANOVA in association with an LSD multiple comparison was used for calculating statistical significance ( A ).

    Journal: Theranostics

    Article Title: Hyperglycemia in combination with excess fat intake promotes renal pyroptosis and fibrosis through Gα 12 -dependent endoplasmic reticulum stress

    doi: 10.7150/thno.124015

    Figure Lengend Snippet: Gα 12 family overexpression in the kidney. (A) Immunodetection of Gα 12 , Gα 13 , PGC1α, and PPARα was carried out on the homogenates used in Figure , with band intensity quantification provided for n= 4 or 5 per group. (B) Representative IHC images showing Gα 12 and Gα 13 localization; scale bar represents 100 μm. (n= 4 or 5 per group) (C) Pearsons's correlation were calculated to determine the correlation between Gα 12 or Gα 13 and either IRE1α or ATF6 (n= 4 or 5/group). For A , β-actin was used as normal control for densitometric analysis. Values are represented as mean ± SEM (** p < 0.01, *** p < 0.001, and N.S., not significant). One-way ANOVA in association with an LSD multiple comparison was used for calculating statistical significance ( A ).

    Article Snippet: Primary antibodies were sourced from various suppliers as follows: α-SMA (ab7817) was obtained from Abcam (Cambridge, MA, USA); Fibronectin (610078) from BD Biosciences (Franklin Lakes, NJ, USA); and activating transcription factor 6 (ATF6) (NBD1-40256) from Novus Biologicals (Littleton, CO, USA).

    Techniques: Over Expression, Immunodetection, Control, Comparison

    Effects of STZ treatments on ER stress, pyroptosis and fibrosis markers in the kidney. (A) A schematic explanation of an animal experiment showing mice subjected to STZ treatments (50 mg/kg body weight, i.p., 3 times on 1 st week and sacrificed after 15 th week). ( B ) Serum RBS, CRE, and BUN contents (n = 5 or 10/group). ( C ) Immunodetection assays for IRE1α, ATF6 and Gα 12 . ( D ) Immunodetection assays for mature IL-1β, Casp-1 and c-Casp-1. ( E ) Immunodetection assays for Col1A1, α-SMA and fibronectin of the same samples. ( F ) PAS staining of the kidney. Scale bar: 100 μm. ( G ) The bar graph shows the glomerular/bowman's capsule ratio of the kidney (n = 5 or 10/group). Each point represents the mean value of 15 arbitrary visual fields in each kidney sample. For comparison of band intensities within the blots, 5 or 9 samples were chosen for immunoblottings based on blood sugar content. ( H ) Pearsons's correlation assay between Gα 12 and IRE-1α was performed as in Fig. 2C (n = 5 or 9/group). For C, D , and E, β-actin was used as normal control for densitometric analysis. For D and E , β-actin is shared. The quantification of band intensities is given to their corresponding samples (n = 5 or 9/group). Values are represented as mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001, and N.S., not significant). Two-tailed unpaired Student's t test was done for calculating statistical significance ( B-E, G ).

    Journal: Theranostics

    Article Title: Hyperglycemia in combination with excess fat intake promotes renal pyroptosis and fibrosis through Gα 12 -dependent endoplasmic reticulum stress

    doi: 10.7150/thno.124015

    Figure Lengend Snippet: Effects of STZ treatments on ER stress, pyroptosis and fibrosis markers in the kidney. (A) A schematic explanation of an animal experiment showing mice subjected to STZ treatments (50 mg/kg body weight, i.p., 3 times on 1 st week and sacrificed after 15 th week). ( B ) Serum RBS, CRE, and BUN contents (n = 5 or 10/group). ( C ) Immunodetection assays for IRE1α, ATF6 and Gα 12 . ( D ) Immunodetection assays for mature IL-1β, Casp-1 and c-Casp-1. ( E ) Immunodetection assays for Col1A1, α-SMA and fibronectin of the same samples. ( F ) PAS staining of the kidney. Scale bar: 100 μm. ( G ) The bar graph shows the glomerular/bowman's capsule ratio of the kidney (n = 5 or 10/group). Each point represents the mean value of 15 arbitrary visual fields in each kidney sample. For comparison of band intensities within the blots, 5 or 9 samples were chosen for immunoblottings based on blood sugar content. ( H ) Pearsons's correlation assay between Gα 12 and IRE-1α was performed as in Fig. 2C (n = 5 or 9/group). For C, D , and E, β-actin was used as normal control for densitometric analysis. For D and E , β-actin is shared. The quantification of band intensities is given to their corresponding samples (n = 5 or 9/group). Values are represented as mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001, and N.S., not significant). Two-tailed unpaired Student's t test was done for calculating statistical significance ( B-E, G ).

    Article Snippet: Primary antibodies were sourced from various suppliers as follows: α-SMA (ab7817) was obtained from Abcam (Cambridge, MA, USA); Fibronectin (610078) from BD Biosciences (Franklin Lakes, NJ, USA); and activating transcription factor 6 (ATF6) (NBD1-40256) from Novus Biologicals (Littleton, CO, USA).

    Techniques: Immunodetection, Staining, Comparison, Two-Photon Excitation Fluorescence Cross-Correlation Assay, Control, Two Tailed Test